HEV Mosaic-S
Hepatitis E Virus Mosaic-S Recombinant

Introduction:
Hepatitis E virus (HEV), the major etiologic agent of enterically transmitted non-A, non-B hepatitis worldwide, is a spherical, non-enveloped, single stranded RNA virus that is approximately 32 to 34 nm in diameter. HEV belongs to a genus of HEV-like viruses (unassigned genus). HEV has a single-stranded polyadenylated RNA genome of approximately 8 kb. Based on its physicochemical properties it is presumed to be a calici-like virus.

Description:
The e.coli derived HEV Mosaic-S protein contains 4 HEV immunodominant regions from the ORF2 and ORF3 having a total molecular mass of 33 kDa. The 4 HEV regions is a part of the 12 HEV regions. HEVMosaic-S comprises of epitope N- terminus of ORF2 and epitope of approximately 500±100aa of ORF3.

Purification Method:
HEV Mosaic-S was purified by proprietary chromatographic technique.

Purity:
HEV Mosaic-S protein is >95% pure as determined by 10% PAGE (coomassie staining).

Formulation:
25mM Tris-HCl, 1mM EDTA, 0.5M urea and 50% glycerol.

Storage:
HEV Mosaic-S protein is shipped at ambient temperature.
Upon arrival, Store at -20°C.

Stability:
5 years frozen. 1 month at 4 room temperature.

Specificity:
Immunoreactive with sera HEV-infected individuals.

Applications:
HEV Mosaic -S antigen is suitable ELISA and Western blots, excellent antigen for detection of HEV with minimal specificity problems.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.