HCV Core Genotype-2a
Hepatitis C Virus Core Genotype-2a Recombinant

Introduction:
HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae.
HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6).

Description:
The E.coli derived recombinant protein contains the HCV core nucleocapsid immunodominant regions, amino acids 2-119.

Purification Method:
HCV Core Genotype-2a protein was purified by proprietary chromatographic technique.

Purity:
HCV Core Genotype-2a protein is >95% pure as determined by 10% PAGE (coomassie staining).

Formulation:
50mM Tris-HCl, pH-8, 60mM NaCl, 10mM glutathione, 0.25% sarkosil and 50% glycerol

Storage:
HCV Core Genotype-2a protein is shipped at ambient temperature.
Upon arrival, Store at -20°C.

Stability:
Five years frozen. One month in solution at room temperature.

Specificity:
Immunoreactive with sera of HCV-infected individuals.

Applications:
HCV Core Genotype-2 antigen is suitabe for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.