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Chorionic gonadotropin beta polypeptide, CGB3, hCGB, Choriogonadotropin subunit beta, CG-beta, Chorionic gonadotrophin chain beta.
Human chorionic gonadotropin (hCG) is a peptide hormoneproduced in pregnancy, that is made by the embryosoon after conceptionand later by the syncytiotrophoblast(part of the placenta). Its role is to prevent the disintegration of the corpus luteumof the ovaryand thereby maintain progesteroneproduction that is critical for a pregnancyin humans. hCG may have additional functions, for instance it is thought that it affects the immune tolerance of the pregnancy. Early pregnancy testinggenerally is based on the detection or measurement of hCG.
Human Chorionic Gonadotropin is produced from a sterile preparation of placental glucoprotein urine of post-menopausal women having a total molecular mass of 36,700 Dalton.CG-beta consists of 237 amino acids, a chain-92 amino acids and b chain-145 amino acids.
The hCG is purified by proprietary chromatographic techniques.
Urine of post-menopausal women
Sterile Filtered White lyophilized (freeze-dried) powder.
The hCG was lyophilized with no additives.
It is recommended to reconstitute the lyophilized Human Chorionic Gonadotropin in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized hCG although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CG-beta should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
The activity was found to be 5000 IU/mg (IRP reference preparation 75/551).
Free of: HbsAg, Hepatitis B surface antigen and antibodies to HIV, Hepatitis C and HCV.
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of hCG as a Reference Standard.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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