HBeAg | ProSpec

Description

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Hepatitis B Virus e-Antigen Recombinant

	5µg($50)
	25µg($130)
	1000µg($2700)

Ice Packs

Catalogue Number

HBV-272

Introduction

Hepatitis B virus is the main cause for human liver disease, chronic infection frequently causes liver cancer and cirrhosis. The HBV core gene codes 2 distinct protein products, a 21.5-kDa protein being assembled to form nucleocapsid particles designated HBcAg, which wraps the viral DNA as well as the viral polymerase and RNase H, and a precore protein, designated as HBeAg, which is directly to the endoplasmic reticulum, processed at N- and C-terminally and secreted as non-particulate e-anitgen (HBeAg). The pre-core protein contains an extra 29 N-terminal amino acids, serving as a signal peptide to direct the nascent polypeptide into secretory pathway. After the secretion, mature HBeAg is deleted at the residue 149 C-terminally and retains 10 precore residues N-terminally. HBcAg and HBeAg are distinctly recognized by antibodies but highly cross-reactive at the T-cell level.
The e antigen is found in the circulation, it is found during the active HBV infection, positive result indicates the risk for contagiousness, and is also used as indicator for the effectiveness of HBV treatment. Positive anti-HBeAg specifies an active stage of acute HBV infection that is in its final stages, the risk for contagiousness is dramatically reduced.

Description

Recombinant e antigen consists of HBV e immunodominant region containing 10 precore residues at its N-terminus and 1-149 residues from the end of precore to its C-terminus. HBV e-Antigen was expressed from E.coli, the purified recombinant core antigen can be used for ELISA assay and Western blot for clinical study and research.

Purification Method

Purified by proprietary chromatographic technique.

Purity

Protein is >95% pure as determined by 10% PAGE (coomassie staining).

Formulation

20mM sodium chloride phosphate buffer, 2M urea.

Storage

HBeAg although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.

Specificity

Immunoreactive with sera HBV-infected individuals.

Applications

Antigen in ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.