HBV core (1-183 a.a.)
Hepatitis B Virus Core (1-183 a.a.) Recombinant

Introduction:
Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.

Description:
The E.Coli derived 18 kDa recombinant protein contains the HBV core ayw immunodominant region, amino acids 1-183.

Purification Method:
HBV Core protein was purified by proprietary chromatographic technique.

Purity:
HBV Core protein is >95% pure as determined by 10% PAGE (coomassie staining).

Formulation:
7.5mM Na-PO4 pH-7.2, 75mM NaCl and 50% glycerol.

Storage:
HBV Core rotein is shipped at ambient temperature.
Upon arrival, Store at -20°C .

Stability:
Five years frozen. One month in solution at room temperature.

Specificity:
Immunoreactive with sera HBV-infected individuals.

Applications:
HBV Core antigen is stable in ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.