|
Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA(pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.
The E.coli derived recombinant protein contains the HBV core immunodominant region amino acids 1-186.
HBV Core protein was purified by proprietary chromatographic technique.
HBV Core protein is >90% pure as determined by 10% PAGE (Coomassie staining).
10mM Tris-HCl pH-8.0, 50mM NaCl, 1mM EDTA & 50% glycerol.
HBV Core protein is shipped at ambient temperature.
Upon arrival, Store at -20°C.
Five years frozen. One month in solution at room temperature.
Immunoreactive with sera HBV-infected individuals.
HBV Core antigen is suitablr for ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
|
