HBV HBe
Hepatitis B Virus HBe Recombinant

Introduction:
Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.
 
Description:
The E.Coli derived 43.7 kDa recombinant protein contains the HBV HBe adw immunodominant region.

Purification Method:
Purified by proprietary chromatographic technique.

Purity:
HBV HBe protein is >95% pure as determined by 10% PAGE (coomassie staining).

Formulation:
25mM Tris-HCl pH 8.0, 1.5 M urea and 50% glycerol.

Storage:
HBV HBe protein is shipped at ambient temperature.
Upon arrival, Store at -20°C.

Stability:
Five years frozen. One month in solution at room temperature.

Specificity:
Immunoreactive with sera HBV-infected individuals.

Applications:
HBV HBe antigen is suitable for ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.