PLA2 IB Human
Secreted Phospholipase A2-IB Human Recombinant

 Synonyms:
Phospholipase A2, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, Group IB phospholipase A2, PLA2, PLA2A, PPLA2, sPLA2-IB,MGC119834, MGC119835.

 Introduction:
Group IB secretory phospholipase A2 (sPLA2-IB) mediates cell proliferation, cell migration, hormone release and eicosanoid production via its receptor in peripheral tissues. In the CNS, high-affinity binding sites of sPLA2-IB have been documented. sPLA2-IB induced neuronal cell death in a concentrationdependent manner depending on PGD2 metabolites, especially Delta12-PGJ2 that might mediate sPLA2-IB-induced apoptosis. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.

 Description:
Secreted Phospholipase A2-IB Human Recombinant is manufactured with N-terminal fusionf HisTag. sPLA2-IBHis-Tagged Fusion Protein is 16 kDa containing 126 amino acid residues of the human secreted phospholipase A2-IB and 16 additional amino acid residues - HisTag (underlined).
MRGSHHHHHH GMASHMAVWQ FRKMIKCVIP GSDPFLEYNN YGCYCGLGGS GTPVDELDKC CQTHDNCYDQ AKKLDSCKFL LDNPYTHTYS YSCSGSAITC SSKNKECEAF ICNCDRNAAI CFSKAPYNKA HKNLDTKKYC QS

 Source:
Escherichia Coli.

 Physical Appearance:
Sterile Filtered lyophilized (freeze-dried) powder.

 Formulation:
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

 Solubility:
Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/ml. In higher concentrations the solubility of this antigen is limited.

 Stability:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C.

 Specificity:
The amino acid sequence of the recombinant human Secreted Phospholipase A2-IB is 100% homologous to the amino acid sequence of the human Secreted
Phospholipase A2-IB.

 Purification Method:
Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.

 Purity:
Greater than 95% as determined by SDS-PAGE.

 Applications:
Western blotting

 References:
• Juhl K, Efanov AM, Olsen HL, Gromada J. Secretory phospholipase A2 is released from pancreatic beta-cells and stimulates insulin secretion via inhibition of ATP-dependent K+ channels. Biochem Biophys Res Commun. 2003 Oct 17; 310(2): 274-9.
• Beck GCh, Yard BA, Schulte J, Haak M, van Ackern K, van der Woude FJ, Kaszkin M. Secreted phospholipases A2 induce the expression of chemokines in microvascular endothelium. Biochem Biophys Res Commun. 2003 Jan 17; 300(3): 731-7.
• Hanasaki K, Arita H. Phospholipase A2 receptor: a regulator of biological functions of secretory phospholipase A2. Prostaglandins Other Lipid Mediat. 2002 Aug; 68-69: 71-82. Review.
• Silliman CC, Moore EE, Zallen G, Gonzalez R, Johnson JL, Elzi DJ, Meng X, Hanasaki K, Ishizaki J, Arita H, Ao L, England KM, Banerjee A. Presence of the M-type sPLA(2) receptor on neutrophils and its role in elastase release and adhesion. Am J Physiol Cell Physiol. 2002 Oct; 283(4): C1102- 13.
• Yagami T, Ueda K, Asakura K, Hayasaki-Kajiwara Y, Nakazato H, Sakaeda T, Hata S, Kuroda T, Takasu N, Hori Y. Group IB secretory phospholipase A2 induces neuronal cell death via apoptosis. J Neurochem. 2002 May; 81(3): 449-61.

 Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.