GPC4 Antibody | ProSpec

Description

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Quantity

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Glypican-4, Mouse Anti Human

	5µg($50)
	20µg($130)
	100µg($450)

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Catalogue Number

ANT-008

Synonyms

Glypican-4, K-glypican, GPC4.

Type

Mouse Anti Human Monoclonal.

Introduction

The glypican family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated manner. The glypican 4 expression is seen in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Hence it is not detected in the midline of the embryo at the stage of intiation of neural tube closure, signifying that glypican-4 is unlikely to play a critical role in convergent extension in the mouse.

Clone

PAT4A3AT.

Immunogen

Anti-human GPC4 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GPC4 amino acids 401-529 purified from E. coli.

Ig Subclass

Mouse IgG1 heavy chain and κ light chain.

Purification Method

GPC4 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Protein formulation

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

Storage Procedures

For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.

Stability / Shelf Life

12 months at -20°C. 1 month at 4°C.

Applications

GPC4 antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500.
Recommended starting dilution is 1:500.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.