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Catalogue Number
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CYT-340
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Synonyms
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Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.
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Introduction
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FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
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Description
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Flt3-Ligand Mouse Recombinant produced in E.Coli is non-glycosylated, polypeptide chain containing 163 amino acids and having a molecular mass of 18587 Dalton.
Flt3-Ligand is purified by proprietary chromatographic techniques.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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The FLT3L Mouse protein was lyophilized with 10mM Na2PO4 pH-7.5.
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Solubility
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It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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Stability
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Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt-3 Ligand should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
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Purity
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Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
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Amino acid sequence
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MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ.
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Biological Activity
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The activity is determined by the ability to generate CD11c+ splenic dendritic cells in vivo in C57B1/6-12 week female mice. To obtain the desired affect, mice were treated with approximately 25ug of flt3-ligand once daily for 9 consecutive days via intraperitoneal injection.
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Usage
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ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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Ness-Ziona, 74140 Israel
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Website : www.prospecbio.com
Email: info@prospecbio.com |
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