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Catalogue Number
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ANT-221
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Type
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Mouse Antibody Monoclonal.
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Introduction
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A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M1 binds the peptide in the presence of bivalent metal cations preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide.
The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
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Clone
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PFLAGHRPSHG.
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Description
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Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH.
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Ig Subclass
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Mouse IgG2b.
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Formulation
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1×PBS & 50% glycerol.
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Stability / Shelf Life
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2 years at -20°C. 1 month at 4°C.
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Dilution
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Western Blotting: 1:4000.
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Applications
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Western Blot.
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Usage
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ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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| Contact Us |
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Toll Free Phone: 1-(866)-245-0885
Toll Free Fax: 1-(866)-796-4944
Postal Address: PO Box 6591,
East Brunswick, 08816 NJ |
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Toll Free Phone: 00-800-800-66666
Toll Free Fax: 00-800-800-67890 |
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International Phone: +972-8-9471175
International Fax: +972-8-9460534
Postal Address: PO Box 4157,
Ness-Ziona, 74140 Israel
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Website : www.prospecbio.com
Email: info@prospecbio.com |
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