FABP5 Human
Epidermal Fatty Acid Binding Protein Human Recombinant

Synonyms:
Fatty acid-binding protein epidermal, E-FABP, Fatty acid-binding protein 5, Psoriasis-associated fatty acid-binding protein homolog, PA-FABP, FABP5, EFABP, PAFABP.

Introduction:
Human Fatty Epidermal Acid Binding Protein FABP also called FABP-5 is a 15 kD member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds ( bile acids or retinoids). In an internal cavity. The fatty acid binding proteins aP2 (fatty acid binding protein [FABP]-4) and mal1 (EFABP) are closely related and both are expressed in adipocytes. Absence of EFABP/mal1 resulted in increased systemic insulin sensitivity in two models of obesity and insulin resistance. Adipocytes isolated from mal1-deficient mice also exhibited enhanced insulin-stimulated glucose transport capacity. In contrast, mice expressing high levels of mal1 in adipose tissue display reduced systematic insulin activity.

Description:
Recombinant Human Epidermal Fatty Acid Binding Protein (FABP-5) is a monodimeric, non-glycosylated, polypeptide chain containing 135 amino acids and having a total molecular mass of 15200 Daltons.

Source:
Escherichia Coli.

Formulation:
Sterile filtered and lyophilized from 0.5 mg/ml in phosphate buffered saline.

Solubility:
Add 0.2 ml of dH₂0 and let the lyophilized pellet dissolve completely.

Stability:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid freeze-thaw cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.

Purity:
Greater than 90% as determined by SDS PAGE.

Specificity:
The amino acid sequence of the recombinant human FABP5 is 100% homologous to the amino acid sequence of the human FABP-5.

Purification Method:
Two-step procedure using size exclusion chromatography before and after refolding.

Applications:
Western blotting, ELISA