Enterokinase Bovine

Description

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Enteropeptidase/Enterokinase Light Chain Bovine Recombinant

	20IU($100)
	100IU($350)
	500IU($1155)

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Catalogue Number

ENZ-311

Synonyms

Enteropeptidase, EC 3.4.21.9, Enterokinase, Serine protease 7, ENTK, MGC133046.

Introduction

Enteropeptidase or enterokinase is an enzymeinvolved in human digestion. It is produced by cells in the duodenum wall, and is secreted from duodenum's glands, the crypts of Lieberk?hn, whenever ingested food enters the duodenum from the stomach. Enteropeptidase has the critical job of turning trypsinogen(a zymogen) to trypsin, indirectly activating a number of pancreaticdigestive enzymes.
Enteropeptidase is a serine proteaseenzyme(EC3.4.21.9). Enteropeptidase is a part of the Chymotrypsin-clan of serine proteases, and is structurally similar to these proteins.

Description

Enterokinase (rEK) Bovine Recombinant is the catalytic subunit of bovine enterokinase, which is expressed by the yeast and purified to yield a high enzyme activity preparation. EK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this sequence.
Recombinant Bovine Enterokinase is a single glycosylated polypeptide chain containing 235 amino acids and having an MW of ~43kDa.

Source

Pichia pastoris.

Physical Appearance

Sterile liquid solution.

Formulation

Bovine EK in 50mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.

Stability

One year when stored at –20°C.
Please avoid freeze-thaw cycles.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Unit Defenition

One unit is defined as the amount of enzyme needed to cleave 50µg of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25mM Tris-HCl, pH 7.6, 50mM NaCl, and 2mM CaCl2.

Assay Conditions

Each reaction contains 50 µg of partially purified thioredoxin-NP-27 fusion protein and varying amounts of Bovine EK. Reactions were incubated at 25°C for 16 hours and analyzed on a Coomassiestained 15% SDS gel.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.