EIF1AY Human | ProSpec

Description

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Quantity

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Eukaryotic Translation Initiation Factor 1A Y-linked Recombinant Human

	2µg($50)
	10µg($130)
	1mg($5200)

Ice Packs

Catalogue Number

PRO-098

Synonyms

Eukaryotic translation initiation factor 1A Y chromosome, Eukaryotic translation initiation factor 4C, eIF-4C, eIF-1A Y isoform.

Introduction

EIF1AY is comparable to eukaryotic translation initiation factor 1A (EIF1A). EIF1AY protein is essential for highest rate of protein biosynthesis. EIF1AY increases ribosome dissociation into subunits and is obligatory for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.

Description

EIF1AY produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-144.a.a) and having a molecular mass of 18.8kDa.
EIF1AY is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The EIF1AY protein solution (0.5mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT and 20% glycerol.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSSMNPEYDY LFKLLLIGDS GVGKSCLLLR FADDTYTESY ISTIGVDFKI RTIELDGKTI KLQIWDTAGQ ERFRTITSSY YRGAHGIIVV YDVTDQESFN NVKQWLQEID RYASENVNKL LVGNKCDLTT KKVVDYTTAK EFADSLGIPF LETSAKNATN VEQSFMTMAA EIKKRMGPGA TAGGAEKSNV KIQSTPVKQS GGGCC

Purity

Greater than 95% as determined by SDS-PAGE.

Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.