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Epidermal growth factor receptor, EC 2.7.10.1, Receptor tyrosine-protein kinase ErbB-1, ERBB, mENA, ERBB1, EGFR.
The epidermal growth factor receptor (EGF R) subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoprotein that has an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand-binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGF R gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGF R has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-á , betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 in the absence of a co-receptor. Ligand binding induces EGF R homodimerization as well as heterdimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGF R can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGF R signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGF R signaling has also been shown to play a role in carcinogenesis.
EGFR Human Recombinant encoding a.a. 672-1210 expressed in Baculovirus infected Sf9 cells.
EGFR is purified by proprietary chromatographic techniques.
Baculovirus infected Sf9 cells
Sterile filtered liquid.
EGFR (0.16 mg/ml) in 50mM Tris-HCl, pH 8.0, 100mM NaCl, 5mM DTT, 15mM reduced glutathione and 20% glycerol.
Store vial at -20ºC to -80ºC. When stored at the recommended temperature, this protein is stable for 12 months.
Please avoid freeze-thaw cycles.
• ELISA
• Inhibition Assays
• Western Blotting
Determination of Km value by Filter binding assay MAFC membrane.
Specific activity: 23.000 pmol/mg min.
On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 91 kDa.
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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