EBV EBNA1 Antibody

Description

Our Price

Quantity

Shipping

EBV EBNA1, Mouse Anti Human

	5µg($50)
	20µg($130)
	0.1mg($450)

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Catalogue Number

ANT-231

Type

Mouse Anti Human Monoclonal.

Introduction

The Epstein-Barr virus (EBV), also called Human herpes virus 4 (HHV-4), is a virus of the herpes family (which includes Herpes simplex virus and Cytomegalovirus. On infecting the B-lymphocyte, the linear virus genome circularizes and the virus subsequently persists within the cell as an episome. The virus can execute several distinct programs of gene expression which can be broadly categorized as being lytic cycle or latent cycle. The lytic cycle or productive infection results in staged expression of a host of viral proteins with the ultimate objective of producing infectious virions. Formally, this phase of infection does not inevitably lead to lysis of the host cell as EBV virions are produced by budding from the infected cell. The latent cycle (lysogenic) programs are those that do not result in production of virions. A very limited, distinct set of viral proteins are produced during latent cycle infection. These include Epstein-Barr nuclear antigen (EBNA)-1, EBNA-2, EBNA-3A, EBNA-3B, EBNA-3C, EBNA-leader protein (EBNA-LP) and latent membrane proteins (LMP)-1, LMP-2A and LMP-2B and the Epstein-Barr encoded RNAs (EBERs).

Clone

P1B5AT.

Immunogen

EBNA1 antibody is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant Epstein-Barr virus nuclear antigen 1 protein.

Ig Subclass

Mouse IgG1 heavy chain and ? light chain.

Purification Method

EBNA1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Protein formulation

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

Storage Procedures

For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.

Stability / Shelf Life

Two years at -20°C. 1 month at 4°C.

Applications

EBNA1 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution ranges for Western blot analysis is 1:1,000 ~ 1:2,000.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.