DKK1 Human | ProSpec

Description

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Quantity

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Dickkopf-Related Protein 1 Human Recombinant

	2µg($180)
	5µg($300)
	10µg($480)

Ice Packs

Catalogue Number

PRO-673

Synonyms

DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK.

Introduction

DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions and takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid and is significantly involved in the development of tau phosphorylation.
DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer.

Description

DKK1 produced in Hi-5 is a single, glycosylated polypeptide chain containing 253 amino acids (32-266 a.a.) and having a molecular mass of 27.8 kDa.
DKK1 is expressed with a 15 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Source

Hi-5 Baculovirus.

Physical Appearance

Sterile filtered colorless solution.

Formulation

The DKK1 protein solution (0.25mg/ml) contains PBS (pH 7.4) and 10% Glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Amino Acid Sequence

ADPTLNSVLN SNAIKNLPPP LGGAAGHPGS AVSAAPGILY PGGNKYQTID NYQPYPCAED EECGTDEYCA SPTRGGDAGV QICLACRKRR KRCMRHAMCC PGNYCKNGIC VSSDQNHFRG EIEETITESF GNDHSTLDGY SRRTTLSSKM YHTKGQEGSV CLRSSDCASG LCCARHFWSK ICKPVLKEGQ VCTKHRRKGS HGLEIFQRCY CGEGLSCRIQ KDHHQASNSS RLHTCQRHSG RLVPRGSHHH HHH.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.