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Chorionic gonadotropin beta polypeptide, CGB3, hCGB,Choriogonadotropin subunit beta, CG-beta, Chorionic gonadotrophin chain beta.
Human chorionic gonadotropin (hCG) is a peptide hormoneproduced in pregnancy, that is made by the embryosoon after conceptionand later by the syncytiotrophoblast(part of the placenta). Its role is to prevent the disintegration of the corpus luteumof the ovaryand thereby maintain progesteroneproduction that is critical for a pregnancyin humans. hCG may have additional functions, for instance it is thought that it affects the immune tolerance of the pregnancy. Early pregnancy testinggenerally is based on the detection or measurement of hCG.
Human Chorionic Gonadotropin Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, glycoprotein chain consisting of two non-covalently linked subunits - designated a and b consisting of 92 and 145 amino acid residues (237 a.a. total, Mw: 36.7kDa), respectively, with carbohydrate moieties linked to ASN-52 and ASN-78 (on a subunit) and ASN-13, ASN-30, SER-121, SER-127, SER-132 and SER-138 (on b subunit). The primary structure of the a chain of r-hCG is identical to that of the a chain of hCG, FSH and LH.
The glycoform pattern of the a subunit of r-hCG is closely comparable to urinary derived hCG (u-hCG), the differences mainly being due to the branching and sialylation extent of the oligosaccharides. The b chain has both O and N-glycosylation sites and its structure and glycosylation pattern are also very similar to that of urinary hCG.
The hCG is purified by proprietary chromatographic techniques.
Chinese Hamster Ovary Cells (CHO).
Sterile Filtered White lyophilized (freeze-dried) powder.
The hCG was lyophilized from a concentrated (1 mg/ml) solution containing 105 mg sucrose and 3.43 phosphoric acid.
It is recommended to reconstitute the lyophilized Human Chorionic Gonadotropin in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized hCG although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CG-beta should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
The biological activity of choriogonadotropin alfa is determined by using the seminal vesicle weight gain test in male rats described in the "Chorionic Gonadotrophins" monographof the European Pharmacopoeia. The in vivo biological activity of choriogonadotropin alfa has been calibrated against the third international reference preparation IS75/587 for chorionic gonadotropin.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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