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GSE, CD152, IDDM12, CELIAC3, CTLA-4.
CTLA-4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Cytotoxic T-Lymphocyte Associated Antigen-4 Human Recombinant produced in insect cells is a homodimeric, glycosylated, polypeptide chain containing a total molecular mass of 80 kDa. Each subunit (40kDa) is fused to a polypeptide linker to the Fc portion of Human IgG1. CTLA4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo.
Large doses of this soluble protein also suppress responses to a second immunization. CTLA-4 is purified by proprietary chromatographic techniques.
Sf9 cells
Sterile Filtered White lyophilized (freeze-dried) powder.
CTLA-4 was lyophilized with no additives.
It is recommended to reconstitute the lyophilized Cytotoxic T-Lymphocyte Associated Antigen-4 Human in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Cytotoxic T-Lymphocyte Associated Antigen Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTLA-4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
The sequence of the amino acids was determined and was found to be: Lys-Ala-Met-His-Val.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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