CSNK2B Human Active

Description

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Quantity

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Casein Kinase 2 beta Human Recombinant, Active

	3µg($400)
	10µg($800)
	20µg($1500)

Catalogue Number

PKA-209

Synonyms

Casein kinase II subunit beta, CK II beta, Phosvitin, G5a, CK2B, CK2N, CSK2B, MGC138222, MGC138224.

Introduction

The Casein kinase 2 (EC2.7.11.1) is a serine/threonine-selective protein kinasethat is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cyclecontrol, DNA repair, regulation of the circadian rhythmand other cellular processes.
Casein kinase 2 activity has been reported to be activated following Wnt signaling pathwayactivation. A Pertussis toxin-sensitive G proteinand Disheveled appear to be an intermediary between Wnt-mediated activation of the Frizzled receptor and activation of casein kinase 2.
Mice that lack casein kinase 2 alpha prime have a defect in the morphology of developing sperm.

Description

Casein Kinase 2 beta Human Recombinant is a non-glycosylated polypeptide having a molecular mass of 24.9 kDa.
Casein Kinase 2 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

CSNK2B lyophilized from 25mM Tris-HCl, 500mM NaCl, 1mM DTT, 1mM EDTA, protease inhibitor cocktail, pH 8.5.

Solubility

Solubilize in distilled H2O or 50% glycerol.

Stability

Lyophilized CSNK2B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CSNK2B should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Unit definition

1U = 1µmol/min at 37 degree C) using the synthetic peptide RRRDDDSDDD.

Purity

Greater than 95% as determined by SDS-PAGE.

Biological Activity

Specific activity > 1U/mg.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.