- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CKI-273
Synonyms
Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM.
Introduction
The three isoenzymes (MM, MB, and BB) are found in muscle, cardiac and brain tissues. These recombinant proteins are ideal for calibrating diagnostic instruments and researching neuromuscular diseases. Creatine Kinases can be used for indications in many neuromuscular applications. These disorders include cardiac disease, mitochondrial disorders, inflammatory myopathies, myasthenia, polymyositis, McArdle''s disease, NMJ disorders, muscular dystrophy, ALS, hypo and hyperthyroid disorders, central core disease, acid maltase deficiency, myoglobinuria, rhabdomyolysis, motor neuron diseases, rheumatic diseases, and other that create elevated or reduced levels of Creatine Kinases.
Description
Human Creatine Kinase M-Type derived from Human Cardiac Tissue.
Source
Human Cardiac Tissue.
Physical Appearance
Lyophilized Powder
Formulation
The CKM protein was lyophilized from 40mM Tris-HCL, 1mM EDTA, pH 7.5 & 10mM n-Acetyl cysteine.
Stability
Lyophilized CKMM although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Solubility
It is recommended to reconstitute the lyophilized CKMM in sterile distilled water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
1 unit will transfer 1 µmole of phosphate from Creatine phosphate to ATP/minute at 37 degrees Celsius. Measured at 340nm as one equimolar amount of NADH produced by a coupled reaction. The specific activity was measured and found to be > 100U/mg.
Safety Data Sheet
Purity
Greater than 10.0% as visualized by sds-page.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.