CKMB | CKMBITII Human

Description

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Creatine Kinase MB Isoenzyme Type-II Human Recombinant

	10µg($50)
	50µg($130)
	1mg($945)

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Catalogue Number

CKI-270

Synonyms

Creatine Kinase MB Isoenzyme Type-II, CKMBITII, CKMBI, CKMB.

Introduction

CK-MB Type II possesses the naturally occurring carboxy-terminal amino acid lysine.
This occurs during a myocardial infarct (MI or heart attack) when CK-MB Type II is released from damaged heart muscle, and the C-terminal lysine is cleaved in the blood stream, thus creating CK-MB Type I. This difference can be exploited in diagnosis of an MI.

Description

CKMBITII Human Recombinant produced in Pichia Pastoris is a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MB Isoenzyme in ELISA.
The CKMBITII is purified by proprietary chromatographic techniques.

Source

Pichia Pastoris.

Physical Appearance

Sterile Filtered colourless liquid formulation.

Formulation

Each mg of protein contains 20mM Tris-HCl, pH-6.8, 1mM EDTA and 1mM DTT.

Stability

CKMBITII although stable at 15°C for 7 days, should be stored below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity

The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 892 IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,171ng/ml.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.