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Catalogue Number
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PKA-211
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Synonyms
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Casein Kinase 2 Holoenzyme, CK2h.
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Description
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Human recombinant casein kinase 2 holo enzyme containing alpha and beta subunits which were separately expressed in E. coli as non-fusion proteins and purified using several chromatographic steps. The holo enzyme has been reconstituted in the course of the purification and is highly active suitable for labelling CK2 substrates. CK2 holoenzyme is a non-glycosilated polypeptide having a molecular mass of 140 kDa.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered clear solution.
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Formulation
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CK2 Holoenzyme is supplied 0.5mg/1ml in 25mM Tris-HCl, 500mM NaCl, 1mM DTT, 500 µM PMSF, 5% glycerol, pH 8.5.
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Unit Definition
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No protease activity detectable, specific activity > 1.3U/mg
(1U = 1µmol/min at 37 degree C) using the synthetic peptide RRRDDDSDDD.
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Storage Conditions
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Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
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Purity
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Greater than 95% as determined by SDS-PAGE.
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Usage
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Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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| Contact Us |
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Toll Free Phone: 1-(866)-245-0885
Toll Free Fax: 1-(866)-796-4944
Postal Address: PO Box 6591,
East Brunswick, 08816 NJ |
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International Phone: +972-8-9471175
International Fax: +972-8-9460534
Postal Address: PO Box 4157,
Ness-Ziona, 74140 Israel
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Website : www.prospecbio.com
Email: info@prospecbio.com |
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