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Catalogue Number
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PRO-222
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Synonyms
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Cell division cycle associated 8, BOR, hDasraB, FLJ12042, MESRGP, Pluripotent embryonic stem cell-related gene 3 protein, PESCRG3, BOREALIN.
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Introduction
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CDCA8 is a component of the chromosomal passenger complex (CPC). The CPC complex has crucial functions at the centromere in ensuring accurate chromosome placement and isolation and is obligatory for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 protein is needed to direct the CPC to centromeric DNA. Removal of CDCA8 delays mitotic progression and causes kinetochore-spindle to mis-attach.
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Description
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CDCA8 Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 283 amino acids (19-280a.a.) and having a molecular mass of 31.6kDa.
The CDCA8 is purified by proprietary chromatographic techniques.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered colorless solution.
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Formulation
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The CDCA8 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 50% glycerol, 1mM DTT, 1mM EDTA and 0.2M NaCl.
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Stability
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CDCA8 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
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Purity
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Greater than 90.0% as determined by SDS-PAGE.
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Amino Acid Sequence
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MGSSHHHHHH SSGLVPRGSH MRKLASFLKD FDREVEIRIK QIESDRQNLL KEVDNLYNIE ILRLPKALRE MNWLDYFALG GNKQALEEAA TADLDITEIN KLTAEAIQTP LKSAKTRKVI QVDEMIVEEE EEEENERKNL QTARVKRCPP SKKRTQSIQG KGKGKRSSRA NTVTPAVGRL
EVSMVKPTPG LTPRFDSRVF KTPGLRTPAA GERIYNISGN GSPLADSKEI FLTVPVGGGE SLRLLASDLQ RHSIAQLDPE ALGNIKKLSN RLAQICSSIR THK
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Usage
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ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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Ness-Ziona, 74140 Israel
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Website : www.prospecbio.com
Email: info@prospecbio.com |
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