CD8 Antibody
CD8, Mouse Anti-Human

Synonyms:
CD8, MAL, p32

Type:
Mouse Anti Human Monoclonal

Introduction:
CD8 is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 acts as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional CD8 is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
CD8 identifies cytotoxic/suppressor t-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of t-cell mediated killing. CD8 alpha chains binds to class-I MHC molecules alpha-3 domains.

Clone:
hCD8

Ig Subclass:
Mouse IgG2a

Purification Method:
Ion exchange column

Protein concentration:
1mg/ml in PBS (after reconstitution)

Storage Procedures:
Lyophilized: store at 4^oC. After reconstitution, if not intended for use within a month, aliquot and store at -20^oC.

Reconstitution:
Reconstitute with of H₂0. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Stability / Shelf Life:
Two years lyophilized, one month in solution at 4^oC.

Antibody Properties:
Blocking and staining antibody. For staining, use 10µl/10⁶ cells. Titer for blocking T cell activation should be determined by the investigator.

Available Conjugates:
This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody use 5-10µl/10⁶ cells.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.