CD4-biotin Antibody
CD4-Biotinylated, Mouse Anti-Human

Synonyms:
gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4, CD4mut.

Type:
Mouse Anti Human Monoclonal

Introduction:
CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell with either have CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized.

Clone:
hCD4

Ig Subclass:
Mouse IgG2b

Purification Method:
Ion exchange column

Protein concentration:
1mg/ml in PBS (after reconstitution)

Storage Procedures:
Lyophilized: store at 4^oC. After reconstitution, if not intended for use within a month, aliquot and store at -20^oC.

Reconstitution:
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Stability / Shelf Life:
Two years lyophilized, one month in solution at 4^oC.

Antibody Properties:
Blocking and staining antibody. Blocks blinding of HIV to CD4. For staining, use 10 ml/10⁶ cells. Titer for blocking T cell activation should be determined by the investigator.

Available Conjugates:
This antibody is also available conjugated to FITC.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.