CAPN2 | ProSpec

Description

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Quantity

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Bovine Calpain-2 Catalytic Subunit

	2µg($175)
	5µg($270)
	10µg($490)

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Catalogue Number

PRO-330

Synonyms

Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2.

Introduction

Calpain’s activity is attributed to two main isoforms: ?-calpain and m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (?-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. Calpain2 (M-calpain) is believed to be membrane bound and functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2.

Description

CAPN2 consists of an 80 kDa large subunit and a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.

Source

Bovine Myocardium.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT and 50% glycerol, pH 7.4.

Stability

CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 90% as determined by SDS-PAGE.

Applications

This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1µl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1'000).
B- Add 1µg of protein to 1ml of the diluted antibody solution and mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections and incubate for 3 days.
E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.