Aprotinin | BPTI

Description

Our Price

Quantity

Shipping

Aprotinin

	100mg($135)
	250mg($270)
	1gram($900)

Catalogue Number

PRO-285

Synonyms

Pancreatic trypsin inhibitor, Basic protease inhibitor, BPI, BPTI, Aprotinin, AP.

Introduction

Aprotinin inhibits the activity of several proteolytic enzymes such as chymotrypsin, kallikrein, plasmin and trypsin. Aprotinin is present in blood and in most tissues, with a high concentration in lung. Aprotinin inhibits pro-inflammatory cytokine release and maintains glycoprotein homeostasis. In platelets, aprotinin reduces glycoprotein loss (e.g., GpIb, GpIIb/IIIa), while in granulocytes it prevents the expression of pro-inflammatory adhesive glycoproteins (e.g., CD11b).

Description

Aprotinin is a natural proteinase inhibitor polypeptide consisting of fifty-eight amino acids {C 284 H 432 N 84 O 79 S 7} arranged in a single polypeptide chain, cross-linked by three disulfide bridges and having a molecular mass of 6512.

Source

Bovine Lung.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein (1mg/ml) was lyophilized with no additives.

Solubility

It is recommended to reconstitute the lyophilized Aprotinin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Aprotinin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Aprotinin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Unit Definition

1 Unit corresponds to 1 biological kallikrein inhibitor unit (KIU)
1 TIU =1,300 KIU.

Activity

6,300 KIU (Kallikrein Inactivator Units) per mg, 4.85 TIU/mg.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.