BID

BID

BID

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  • View Data Sheet

    Description:

    Truncated BH3 Interacting Domain Death Agonist Mouse Recombinant

    Truncated BH3-interacting domain death agonist, p22 BID, BID, FP497, MGC15319, MGC42355, tBID.

    Product # :

    PRO-644

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    • description
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    Description

    Truncated BID Mouse Recombinant also called BH3-interacting domain death agonist p15 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 61-195 amino acids (135 a.a.) and having a molecular mass of 15.4 kDa.

    Source

    Escherichia Coli.

    Formulation

    The Mouse Truncated BID protein solution contains 10mM Tris-HCl pH-8, 1mM EDTA and 250mM NaCl.

    Purity

    Greater than 95.0% as determined by:
    (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    More Info

    • Introduction

      Truncated BH3 interacting domain death agonist is a truncated form of the pro-apoptotic full-length BID. Truncated BH3 interacting domain death agonist is generated by Caspase-8 cleavage of BID. The truncated form of the protein translocates from the cytosol to mitochondria and transduces apoptotic signals.
      BID is a pro-apoptotic Bcl-2 protein having only the BH3 domain. In reaction to apoptotic signaling, BID interacts with another Bcl-2 family of cell death regulators, called Bax, they form a heterodimer resulting to the insertion of Bax into the outer mitochondrial membrane. Bax induces the opening of the mitochondrial voltage-dependent anion channel which lead to the release of cytochrome c and other pro-apoptotic factors from the mitochondria resulting in activation of caspases. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. The major proteolytic product p15 BID releasea cytochrome c. Isoform 1, Isoform 2 and Isoform 4 induce ice-like proteases and apoptosis while Isoform 3 does not induce apoptosis.

    • Synonyms

      Truncated BH3-interacting domain death agonist, p22 BID, BID, FP497, MGC15319, MGC42355, tBID.

    • Physical Appearance

      Sterile Filtered colorless liquid formulation.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Tbid Mouse
  • View Data Sheet

    Description:

    BH3 Interacting Domain Death Agonist Mouse Recombinant, GST

    BH3-interacting domain death agonist, p22 BID, BID, FP497, MGC15319, MGC42355.

    Product # :

    PRO-643

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    Quantity :

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    Description

    BID Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 1-195 amino acids and having a molecular mass of 48 kDa.The Mouse BID is expressed as GST-Tag fusion protein and purified by proprietary chromatographic technique.

    Source

    Escherichia Coli.

    Formulation

    The Mouse GST tag BID protein solution contains 10mM Tris-HCl pH-8, 1mM EDTA and 250mM NaCl.

    Purity

    Greater than 95.0% as determined by: (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    More Info

    • Introduction

      BID is a pro-apoptotic Bcl-2 protein having only the BH3 domain. In reaction to apoptotic signaling, BID interacts with another Bcl-2 family of cell death regulators, called Bax, they form a heterodimer resulting to the insertion of Bax into the outer mitochondrial membrane. Bax induces the opening of the mitochondrial voltage-dependent anion channel which lead to the release of cytochrome c and other pro-apoptotic factors from the mitochondria resulting in activation of caspases. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. The major proteolytic product p15 BID releasea cytochrome c. Isoform 1, Isoform 2 and Isoform 4 induce ice-like proteases and apoptosis while Isoform 3 does not induce apoptosis.

    • Synonyms

      BH3-interacting domain death agonist, p22 BID, BID, FP497, MGC15319, MGC42355.

    • Physical Appearance

      Sterile Filtered colorless liquid formulation.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Bid Mouse Gst
  • View Data Sheet

    Description:

    BH3 Interacting Domain Death Agonist Mouse Recombinant

    BH3-interacting domain death agonist, p22 BID, BID, FP497, MGC15319, MGC42355.

    Product # :

    PRO-642

    Price :

    Quantity :

    Shipping Method :

    Ice Icon

    Shipped with Ice Packs

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    More Info

    • description
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    Description

    BID Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 1-195 amino acids and having a molecular mass of 22 kDa.

    Source

    Escherichia Coli.

    Formulation

    The Mouse BID protein solution contains 10mM Tris-HCl pH-8, 1mM EDTA and 250mM NaCl.

    Purity

    Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

    More Info

    • Introduction

      BID is a pro-apoptotic Bcl-2 protein having only the BH3 domain. In reaction to apoptotic signaling, BID interacts with another Bcl-2 family of cell death regulators, called Bax, they form a heterodimer resulting to the insertion of Bax into the outer mitochondrial membrane. Bax induces the opening of the mitochondrial voltage-dependent anion channel which lead to the release of cytochrome c and other pro-apoptotic factors from the mitochondria resulting in activation of caspases. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. The major proteolytic product p15 BID releasea cytochrome c. Isoform 1, Isoform 2 and Isoform 4 induce ice-like proteases and apoptosis while Isoform 3 does not induce apoptosis.

    • Synonyms

      BH3-interacting domain death agonist, p22 BID, BID, FP497, MGC15319, MGC42355.

    • Physical Appearance

      Sterile Filtered colorless liquid formulation.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Bid Mouse
  • View Data Sheet

    Description:

    BH3 Interacting Domain Death Agonist Human Recombinant

    BH3-interacting domain death agonist, p22 BID, BID, FP497, MGC15319, MGC42355.

    Product # :

    PRO-627

    Price :

    Quantity :

    Shipping Method :

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    Shipped with Ice Packs

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    • description
    • source
    • formulation
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    Description

    BID Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 195 amino acids and having a molecular mass of 21.9 kDa.

    Source

    Escherichia Coli.

    Formulation

    The protein solution contains 20mM Tris-HCl pH-8 & 20% NaCl.

    Purity

    Greater than 95.0% as determined by:
    (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    More Info

    • Introduction

      BID accession number NP_001187 is a pro-apoptotic Bcl-2 protein having only the BH3 domain. In reaction to apoptotic signaling, BID interacts with another Bcl-2 family of cell death regulators, called Bax, they form a heterodimer resulting to the insertion of Bax into the outer mitochondrial membrane. Bax induces the opening of the mitochondrial voltage-dependent anion channel which lead to the release of cytochrome c and other pro-apoptotic factors from the mitochondria resulting in activation of caspases. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. The major proteolytic product p15 BID releasea cytochrome c. Isoform 1, Isoform 2 and Isoform 4 induce ice-like proteases and apoptosis while Isoform 3 does not induce apoptosis.

    • Synonyms

      BH3-interacting domain death agonist, p22 BID, BID, FP497, MGC15319, MGC42355.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MDCEVNNGSS LRDECITNLL VFGFLQSCSD NSFRRELDAL GHELPVLAPQ WEGYDELQTD GNRSSHSRLG RIEADSESQE
      DIIRNIARHL AQVGDSMDRS IPPGLVNGLA LQLRNTSRSE EDRNRDLATA LEQLLQAYPR DMEKEKTMLV LALLLAKKVA SHTPSLLRDV FHTTVNFINQ NLRTYVRSLA RNGMD.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Bid Human

About BID / BH3 Interacting Domain Death Agonist:

Defined as a protein-coding gene, BH3 Interacting Domain Death Agonist (BID) is a member of the Bcl-2 protein family. It is pro-apoptotic, meaning that it facilitates cell death or mutation via its impact on various cellular activities.

BID Function
BH3 Interacting Domain Death Agonist counteracts the protective effect of Bcl-2 and induces apoptosis and caspases. The main protease present in BH3 Interacting Domain Death Agonist in p15 BID, which facilitates the release of cytochrome c. Isoform 1, 2, and 4 induce ICE-like proteases and apoptosis, whereas Isoform 3 does not.

BH3 Interacting Domain Death Agonist Interactions
BH3 Interacting Domain Death Agonist interacts with BAX, another member of the Bcl-2 protein family, as well as Serine/threonine-protein kinase ATR, Caspase 2, Caspase-8, Induced myeloid leukemia cell differentiation protein Mcl-1, and Replication protein A (RPA).

BID Mechanism
Upon interaction, BH3 Interacting Domain Death Agonist infiltrates a cell and typically instigates modification or cell death. When BID interacts with BAX, for example, BAX is inserted into the organelle membranes. Subsequently, the mitochondria release cytochrome c, in addition to other pro-apoptotic factors, which, in turn, activates caspases.
If tumor suppressor p53 is present, the expression of BH3 Interacting Domain Death Agonist is upregulated. Due to this, BID is also believed to be involved in p53-mediated apoptosis.
Additionally, studies have shown that BID is often activated in response to particular apoptotic stimuli in a death receptor-independent manner.
However, Bcl-2 and anti-apoptotic proteins within the Bcl-2 family, are capable of inhibiting BID’s ability to activate BAX. As a result, anti-apoptotic Bcl-2 proteins can minimize the impact of BID by binding to BAX.