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Catalogue Number
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ENZ-060
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Synonyms
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3-hydroxybutyrate dehydrogenase type 2, FLJ13261, PRO20933, SDR15C1, UCPA-OR, UNQ6308, dehydrogenase/reductase (SDR family) member 6, Oxidoreductase UCPA, DHRS6, R-beta-hydroxybutyrate dehydrogenase, EFA6R, EC 1.1.1.30.
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Introduction
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BDH2 is a member of the short-chain dehydrogenases/reductases (SDR) family. BDH2 protein has a significant part in the peripheral utilization of 3-hydroxybutyrate. BDH2 can convert high levels of circulating 3-hydroxybutyrate into acetoacetate due to cytoplasmic localization in high ratio of oxidized NAD+, the NAD+ dependence and the kinetic parameters.
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Description
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BDH2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 265 amino acids (1-245a.a.) and having a molecular mass of 28.8kDa. BDH2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered clear solution.
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Formulation
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The BDH2 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 0.1M NaCl and 10% glycerol.
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Amino Acid Sequence
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MGSSHHHHHH SSGLVPRGSH MGRLDGKVII LTAAAQGIGQ AAALAFAREG AKVIATDINE SKLQELEKYP GIQTRVLDVT KKKQIDQFAN EVERLDVLFN VAGFVHHGTV LDCEEKDWDF SMNLNVRSMY LMIKAFLPKM LAQKSGNIIN MSSVASSVKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV DTPSLQERIQ ARGNPEEARN DFLKRQKTGR FATAEEIAML CVYLASDESA YVTGNPVIID GGWSL
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Purity
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Greater than 95% as determined by SDS-PAGE.
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Storage
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Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
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Usage
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ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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| Contact Us |
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Toll Free Phone: 1-(866)-245-0885 Toll Free Fax: 1-(866)-796-4944 Postal Address: PO Box 6591, East Brunswick, 08816 NJ |
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Toll Free Phone: 00-800-800-66666 Toll Free Fax: 00-800-800-67890 |
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International Phone: +972-8-9471175 International Fax: +972-8-9460534 Postal Address: PO Box 4157, Ness-Ziona, 74140 Israel
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Website : www.prospecbio.com Email: info@prospecbio.com |
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