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Protein Catalog >> Enzymes >> Dehydrogenase >> BCOADC-E2 Human
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Description
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2-Oxo-Acid Dehydrogenase Complex E2 Human Recombinant
Ice Packs
Catalogue Number ENZ-084
Description BCOADC-E2 is a Full-length cDNA coding for the mature form of the human BCOADC-E2 protein having a molecular mass of 47,321 Dalton(pH 6.5). BCOADC-E2 protein is fused to a hexa-histidine purification tag.
Source Sf9 insect cells.
Formulation DLST (0.91mg/ml) is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.
Immunological function Binds IgG-type human auto-antibodies.
Immunological tests Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.
Applications Western blot with anti-M2-Antigen autoantibody-positive patient sera or monoclonal
anti-hexa-His-tag antibody.
Coating concentration 0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity Greater than 75% as determined by SDS-PAGE.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
» DLAT Human
» DLST Human
» M2 Human
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