IAPP Human
Amylin Human Recombinant

Synonyms:
DAP, IAP, AMYLIN, islet amyloid polypeptide, Diabetes-associated peptide, Insulinoma amyloid peptide, IAPP.

Introduction:
Amylin, or Islet Amyloid Polypeptide (IAPP), is a 37-residue peptide hormonesecreted by pancreatic β-cellsat the same time as insulin(in a roughly 100:1 ratio).
Amylin functions as part of the endocrinepancreasand contributes to glycemic control. Although amylin's complete function may not yet be known, it has been shown to slow gastric emptying, promote satiety, inhibit secretion of glucagonduring hyperglycemia, and therein reduce the total insulindemand. As insulin lowers blood glucose and glucagon raises blood glucose, amylin supports the stability of blood glucose levels in effect by slowing the rate that digested glucose enters the bloodstream.
Rodent amylin knockoutsare known to fail to achieve the normal anorexiafollowing food consumption. Because it is an amidated peptide, like many neuropeptides, it is believed to be responsible for the anorectic effect.
It was identified independently by two groups as the major component of islet Amyloid deposits in 1987 (Cooper et al and Westermark et al).
There appears to be at least three distinct receptor complexes that bind with high affinity to amylin. All three complexes contain the calcitonin receptorat the core, plus one of three Receptor activity-modifying proteins, RAMP1, RAMP2, or RAMP3.

Description:
Amylin (IAPP: Islet Amyloid PolyPeptide) Human Recombinant produced in E. coli, is a non-glycosylated, Polypeptide chain containing 37 amino acids and having a molecular mass of 3903.4 Dalton.
The Amylin is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
KCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTY (37aa).

Source:
Escherichia Coli.

Formulation:
The Amylin peptide was lyophilized in 1% Acetic acid.

Solubility:
Resuspend in 1 % Acetic acid.

Stability:
Upon arrival store at -20°C.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

References:
1) Deems, R. O. et al. (1991) Biochem. Biophys. Res. Commun. 181: 16
2) Rink TJ, et. al., (1993) Trends Pharmacol Sci 14: 113-118
3) Cooper, G.J. et. al., (1987) Proc Natl Acad Sci USA 84:8628-8632
4) Westermark,P. et. al., (1987) Proc Natl Acad Sci USA 84:3881-3885

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.