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Catalogue Number
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ENZ-275
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Synonyms
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Bacterial leucyl aminopeptidase, EC 3.4.11.10.
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Description
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The 29 kDa Aeromonas Aminopeptidase is produced by genetic engineering and can be used for physical & structural investigations, sequence and amino-terminal determinations. This exopeptidase recognizes a specific stop sign at –X- Pro and requires a free a-amino group in the L-configuration. It is therefore suitable for the removal of the redundant N-terminal methionine often added to engineered recombinant proteins.
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Source
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Aeromonas Proteolytica.
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Physical Appearance
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Sterile filtered liquid formulation.
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Formulation
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Buffered solution containing 10mM Tris-HCL, 100mM NaCl and 5µM ZnSO4, pH 8.0.
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Purity
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Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
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Stability
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Two years when stored at –20°C, 2 weeks at 4°C.
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Unit Defenition
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One unit is defined as the amount of enzyme that hydrolyses 1µmole of L-leucine p-nitroanilide at 25°C per 1 minute.
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Biological Activity
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Recombinant Aeromonas Aminopeptidase was found to have an activity of 120 Units/mg protein.
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Usage
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Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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