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EC 3.1.3.1, IAP, AP.
The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
Calf Intestine
Sterile Filtered clear colorless solution (11.1 mg/ml).
50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0.
AP although stable at 14°C for 1 weeks, should be stored at 4°C.
>1500 U/mg (pH 9.6, 37°C and 0.025M glycin, 10% glycerol as buffer).
95% pure by Gel Filtration.
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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