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Catalogue Number
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ENZ-322
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Synonyms
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EC 3.1.3.1, IAP, AP.
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Description
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The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
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Source
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Calf Intestine.
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Physical Appearance
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Sterile Filtered clear colorless solution.
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Formulation
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50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0.
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Stability
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AP should be stored at 4°C.
Please Do-Not freeze.
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Biological Activity
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>1000 U/mg (pH 9.6, 25°C and 0.025M glycin, 10% glycerol as buffer.
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Purity
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95% pure by Gel Filtration.
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Usage
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Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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Website : www.prospecbio.com
Email: info@prospecbio.com |
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