Adenylate Kinase

Adenylate Kinase

Adenylate Kinase

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  • View Data Sheet

    Description:

    Adenylate Kinase 5 Human Recombinant

    Adenylate kinase isoenzyme 5, AK 5, ATP-AMP transphosphorylase 5, AK5, AK6.

    Product # :

    PKA-011

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    Description

    AK5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 586 amino acids (1-562 a.a.) and having a molecular mass of 65.9kDa.AK5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    AK5 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      Adenylate kinase isoenzyme 5 (AK5) belongs to the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. AK5 is related to the UMP/CMP kinase of several species. AK5 is located in the cytosol and expressed exclusively in the brain.

    • Synonyms

      Adenylate kinase isoenzyme 5, AK 5, ATP-AMP transphosphorylase 5, AK5, AK6.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMNTNDA KEYMARREIP QLFESLLNGL MCSKPEDPVE YLESCLQKVK ELGGCDKVKW DTFVSQEKKT LPPLNGGQSR RSFLRNVMPG NSNFPYRRYD RLPPIHQFSI ESDTDLSETA ELIEEYEVFD PTRPRPKIIL VIGGPGSGKG TQSLKIAERY GFQYISVGEL LRKKIHSTSS NRKWSLIAKI ITTGELAPQE TTITEIKQKL MQIPDEEGIV IDGFPRDVAQ ALSFEDQICT PDLVVFLACA NQRLKERLLK RAEQQGRPDD NVKATQRRLM NFKQNAAPLV KYFQEKGLIM TFDADRDEDE VFYDISMAVD NKLFPNKEAA AGSSDLDPSM ILDTGEIIDT GSDYEDQGDD QLNVFGEDTM GGFMEDLRKC KIIFIIGGPG SGKGTQCEKL VEKYGFTHLS TGELLREELA SESERSKLIR DIMERGDLVP SGIVLELLKE AMVASLGDTR GFLIDGYPRE VKQGEEFGRR IGDPQLVICM DCSADTMTNR LLQRSRSSLP VDDTTKTIAK RLEAYYRASI PVIAYYETKT QLHKINAEGT PEDVFLQLCT AIDSIF.

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    Ak5 Human
  • View Data Sheet

    Description:

    Adenylate Kinase 4 Human Recombinant

    Adenylate Kinase 4, AK3, Adenylate Kinase 3-like 1, AK3L1, ATP-AMP transphosphorylase, Adenylate Kinase 3, GTP:AMP phosphotransferase, mitochondrial adenylate kinase-3, AK3L2, MGC166959, EC 2.7.4.3.

    Product # :

    PKA-267

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    Description

    AK4 Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 243 amino acids (1-223a.a.) and having a molecular mass of 27.4kDa. AK4 protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.

    Source

    Escherichia Coli.

    Formulation

    AK4 Human solution (1mg/1ml) containing 20mM Tris-HCl pH-8, 2mM DTT, 0.1M NaCl, and 10% glycerol.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      AK4 belongs to the adenylate kinase family of enzymes. AK4 protein is restricted to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide structures in a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Hereditary mutations which cause AK4 deficiencies in erythrocytes have been associated to hemolytic anemia.

    • Synonyms

      Adenylate Kinase 4, AK3, Adenylate Kinase 3-like 1, AK3L1, ATP-AMP transphosphorylase, Adenylate Kinase 3, GTP:AMP phosphotransferase, mitochondrial adenylate kinase-3, AK3L2, MGC166959, EC 2.7.4.3.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MASKLLRAVI LGPPGSGKGT VCQRIAQNFG LQHLSSGHFL RENIKASTEV GEMAKQYIEK SLLVPDHVIT RLMMSELENR RGQHWLLDGF PRTLGQAEAL DKICEVDLVI SLNIPFETLK DRLSRRWIHP PSGRVYNLDF NPPHVHGIDD VTGEPLVQQE DDKPEAVAAR LRQYKDVAKP VIELYKSRGV LHQFSGTETN KIWPYVYTLF SNKITPIQSK EAY

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    Ak4 Human
  • View Data Sheet

    Description:

    Adenylate Kinase-3 Like 1 Human Recombinant

    AK3, Adenylate kinase 3 alpha like 1, AK3L1, AK4.

    Product # :

    PKA-346

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    Description

    AK3L1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 259 amino acids (1-223) and having a molecular mass of 29.3 kDa. The AK3L1 is fused with 35 amino acid His-tag at N-terminus.AK3L1 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    AK3L1 solution containing 20mM Tris-HCl pH-8, 2mM DTT and 20% glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      AK3L1 is an affiliate of the adenylate kinase family of enzymes. AK3L1 enzyme is restricted to the mitochondrial matrix. Adenylate kinases-3 regulates the adenine and guanine nucleotide compositions within a cell by means of catalyzing the reversible transfer of phosphate group among these nucleotides. 5 different isozymes of adenylate kinase have been identified in vertebrates. The enzyme expression of various adenylate kinase isozymes is growth regulated and tissue-specific.

    • Synonyms

      AK3, Adenylate kinase 3 alpha like 1, AK3L1, AK4.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASK LLRAVILGPP GSGKGTVSQR IAQNFGLQHL SSGHFLRENIKASTEVGEMA KQYIEKSLLV PDHVITRLMM SELENRRGQH WLLDGFPRTL GQAEALDKIC EVDLVISLNI PFETLKDRLS RRWIHPPSGR VYNLDFNPPH VHGIDDVTGE PLVQQEDDKP EAVAARLRQY KDVAKPVIEL YKSRGVLHQF SGTETNKIWP YVYTLFSNKI TPIQSKEAY.

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    Ak3L1 Human
  • View Data Sheet

    Description:

    Adenylate Kinase 2 Mouse Recombinant

    Adenylate kinase 2 mitochondrial isoform a, Ak-2, D4Ertd220e, mitochondrial, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferas, Adenylate monophosphate kinase.

    Product # :

    PKA-107

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    Description

    AK2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids (1-239 a.a) and having a molecular mass of 29kDa.AK2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    AK2 protein solution (0.5mg/ml) containing 20mM Tris-Hcl buffer (pH8.5), 10% glycerol and 1mM DTT.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    Biological Activity

    Specific activity is > 40 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

    More Info

    • Introduction

      Adenylate kinases play a role in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. There are 3 types of adenylate kinase isozymes, AK1, AK2, and AK3 in vertebrates. Expression of these isozymes are tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and is involved in apoptosis. AK2 is mutated in individuals with reticular dysgenesis.

    • Synonyms

      Adenylate kinase 2 mitochondrial isoform a, Ak-2, D4Ertd220e, mitochondrial, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferas, Adenylate monophosphate kinase.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      AK2 Mouse Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMAPNVL ASEPEIPKGI RAVLLGPPGA GKGTQAPKLA ENFCVCHLAT GDMLRAMVAS GSEL TMDAGKLVSD EMVVELIEKN LETPSCKNGF LLDGFPRTVR QAEMLDDLME KRKEKLDSVI EFSIQDSLLI RRITGRLIHP KSGRS
      NPPKEPMKDD ITGEPLIRRS DDNEKALKTR LEAYHTQTTP LVEYYRKRGI HCAIDASQTP DIVFASILAA FSKATCKDLV MFI

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    Ak2 Mouse
  • View Data Sheet

    Description:

    Adenylate Kinase 2 Human Recombinant

    ADK2, AK-2, Adenylate kinase isoenzyme 2 mitochondrial, ATP-AMP transphosphorylase 2, adenylate kinase 2.

    Product # :

    PKA-260

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    Description

    AK2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 259 amino acids and having a molecular mass of 28.6 kDa. AK2 is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    AK2 solution containing 20mM Tris pH-7.5, 5mM DTT and 20% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    Biological Activity

    Specific activity: > 1.5 units/ml. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 25C.

    More Info

    • Introduction

      Adenylate kinases play a role in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. There are 3 types of adenylate kinase isozymes, AK1, AK2, and AK3 in vertebrates. Expression of these isozymes are tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and is involved in apoptosis. AK2 is mutated in individuals with reticular dysgenesis.

    • Synonyms

      ADK2, AK-2, Adenylate kinase isoenzyme 2 mitochondrial, ATP-AMP transphosphorylase 2, adenylate kinase 2.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      AK2 Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MAPSVPAAEP EYPKGIRAVL LGPPGAGKGTQAPRLAENFC VCHLATGDML RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE KLDSVIEFSIPDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI.

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    Ak2 Human
  • View Data Sheet

    Description:

    Adenylate Kinase 1 Mouse Recombinant

    Adenylate kinase Isoenzyme 1 isoform 1, Ak-1, B430205N08Rik, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Myokinase.

    Product # :

    PKA-106

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    Description

    AK1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (1-210 a.a) and having a molecular mass of 25.5kDa.AK1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    AK1 protein solution (0.5mg/ml) containing 20mM Tris-Hcl buffer (pH8.0), 10% glycerol and 1mM DTT.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    Biological Activity

    Specific activity is > 150 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

    More Info

    • Introduction

      AK1 is a small ubiquitous enzyme which is essential for maintenance and cell growth. It is involved in the regulation of adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. The AK1 protein is found in the cytosol of skeletal muscle, brain and erythrocytes. Defects in the AK1 gene are the cause of a form of hemolytic anemia.

    • Synonyms

      Adenylate kinase Isoenzyme 1 isoform 1, Ak-1, B430205N08Rik, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Myokinase.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDD
      NEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK.

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    Ak1 Mouse
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    Description:

    Adenylate Kinase 1 Human Recombinant

    Adenylate kinase isoenzyme 1, AK 1, ATP-AMP transphosphorylase 1, Myokinase, AK1.

    Product # :

    PKA-316

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    Description

    AK1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 214 amino acids (1-194 a.a.) and having a molecular mass of 23.7kDa. The AK1 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The AK1 protein solution (1mg/ml) contains 20mM Tris-HCl pH-7.5 and 10% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    Biological Activity

    Specific activity: > 600 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 25°C.

    More Info

    • Introduction

      AK1 is a small ubiquitous enzyme which is essential for maintenance and cell growth. It is involved in the regulation of adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. The AK1 protein is found in the cytosol of skeletal muscle, brain and erythrocytes. Defects in the AK1 gene are the cause of a form of hemolytic anemia.

    • Synonyms

      Adenylate kinase isoenzyme 1, AK 1, ATP-AMP transphosphorylase 1, Myokinase, AK1.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK.

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    Ak1 Human
  • View Data Sheet

    Description:

    Adenosine Kinase Mouse Recombinant

    AK, ADK, Adenosine Kinase, Adenosine 5-Phosphotransferase.

    Product # :

    PKA-105

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    Description

    ADK produced in E.Coli is a single, non-glycosylated polypeptide chain containing 384 amino acids (1-361a.a.) and having a molecular mass of 42.5kDa.ADK is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The ADK protein solution (1mg/ml) contains 20% glycerol, 20mM Tris-HCl buffer (pH8.0), 1mM EDTA & 50mM NaCl.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    Biological Activity

    Specific activity is > 100 pmol/min/ug and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH. 

    More Info

    • Introduction

      Adenosine Kinase is an abundant enzyme in mammalian tissues which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thus is as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has extensive effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme take a crucial pharmacological part in growing intravascular adenosine concentrations and acting as anti-inflammatory agents.

    • Synonyms

      AK, ADK, Adenosine Kinase, Adenosine 5-Phosphotransferase.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMAAADEP KPKKLKVEAP QALSENVLFG MGNPLLDISA VVDKDFLDKY SLKPNDQILA EDKHKELFDE LVKKFKVEYH AGGSTQNSMK VAQWLIQEPH KAATFFGCIG IDKFGEILKR KAADAHVDAH YYEQNEQPTG TCAACITGGN RSLVANLAAA NCYKKEKHLD LERNWVLVEK ARVYYIAGFF LTVSPESVLK VARYAAENNR VFTLNLSAPF ISQFFKEALM DVMPYVDILF GNETEAATFA REQGFETKDI KEIAKKAQAL PKVNSKRQRT VIFTQGRDDT IVAAENDVTA FPVLDQNQEE IIDTNGAGDA FVGGFLSQLV SDKPLTECIR AGHYAASVII RRTGCTFPEK PDFH.

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    Adenosine Kinase Mouse
  • View Data Sheet

    Description:

    Adenosine Kinase Human Recombinant, Active

    Adenosine 5'-phosphotransferase, EC 2.7.1.20, AK, ADK, Adenosine Kinase, Adenosine 5-Phosphotransferase, Testicular Tissue Protein Li 14, EC 2.7.1.

    Product # :

    PKA-108

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    Description

    ADK produced in E.Coli is a single, non-glycosylated polypeptide chain containing 362 amino acids (22-362a.a.) and having a molecular mass of 40.5kDa.ADK is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The ADK protein solution (0.5mg/ml) contains 20% glycerol, 20mM Tris-HCl buffer (pH8.0), 1mM DTT, 1mM EDTA & 50mM NaCl.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    Biological Activity

    Specific activity is > 30 pmol/min/ug and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

    More Info

    • Introduction

      Adenosine Kinase is an abundant enzyme in mammalian tissues which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thus is as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has extensive effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme take a crucial pharmacological part in growing intravascular adenosine concentrations and acting as anti-inflammatory agents.

    • Synonyms

      Adenosine 5'-phosphotransferase, EC 2.7.1.20, AK, ADK, Adenosine Kinase, Adenosine 5-Phosphotransferase, Testicular Tissue Protein Li 14, EC 2.7.1.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKAR
      VCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPD
      FH.

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    Adenosine Kinase
  • View Data Sheet

    Description:

    Adenosine Kinase Human Recombinant

    Adenosine 5'-phosphotransferase, EC 2.7.1.20, AK, ADK.

    Product # :

    PKA-367

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    Description

    Adenosine Kinase Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 362 amino acids (22-362a.a.) and having a molecular mass of 40.5 kDa. Adenosine Kinase protein is fused to a 21 amino acid His tag at N-terminus and is purified by standard chromatography.

    Source

    Escherichia Coli.

    Formulation

    ADK protein solution (0.5mg/ml) containing 20mM Tris-HCl pH-8, 1mM DTT, 1mM EDTA, 50mM NaCl & 20% glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      Adenosine Kinase is an abundant enzyme in mammalian tissues which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thus is as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has extensive effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme take a crucial pharmacological part in growing intravascular adenosine concentrations and acting as anti-inflammatory agents.

    • Synonyms

      Adenosine 5'-phosphotransferase, EC 2.7.1.20, AK, ADK.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPD FH.

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    Adk Human

About Adenylate kinase / ADK:

Adenylate kinase is often referred to as the ADK protein. This protein is a phosphotransferase enzyme with catalytic properties. It helps catalyze the interconversion of adenine nucleotides - this includes ATP, ADP, and AMP. In essence, it will offer continuous monitoring of the phosphate nucleotide levels inside of a cell. Therefore, ADK is seen to play one of the critical roles in cellular energy homeostasis.
Adenylate kinase Structure
Adenylate kinase is considered a signal-transducing protein. As a consequence, this affects the way it is structured. It has a balance between conformations, which regulates protein activity. When it isn’t bound to anything, ADK has a locally unfolded state.
Interestingly, studies have shown that ADK protein actually has three different structures. There’s the open structure, the closed structure, and the CORE structure. As such, it is considered to be a very flexible protein. What’s more, ADK is also said to have a crystal structure.

ADK Mechanism & Interactions
Phosphoryl transfer is only possible when the ‘open lid’ of ADK has closed. From here, water molecules can’t get in, which helps move different substrates closer to one another. In turn, this reduces the energy barrier for the nucleophilic attack by the y-phosphoryl group of ATP upon the a-phosphoryl of AMP.
If we look at the structure of the ADK enzyme present in E. coli - with inhibitor Ap5A - then we notice that the Arg88 residue connects to the Ap5A. From here, the mutation of R88G leads to a near 100% loss of catalytic activity for this specific enzyme. As such, it points to the idea that this residue is intrinsically linked to phosphoryl transfer.
On the adenose region of ADK, we have the highly conserved Arg119 residue. Ere, this residue sandwiches the adenine in the active binding site. By doing this, it’s believed that the promiscuity of these enzymes in how they accept other NTP’s is related to the interactions on the base of the ATP binding pocket.

Adenylate kinase Function
There are two critical functions of adenylate kinase. Firstly, we have metabolic monitoring. This refers to a cells ability to measure energetic levels with a method to monitor metabolic processes. ADK continuously monitors ATP, ADP, AMP - and other adenyl phosphates. By doing this, it helps regulate the amount of energy expended at the cellular level. When various metabolic stresses alter the energy levels, ADK can then generate AMP to help control things. ADK activity is commonly influenced by the following; exercise, stress, hormone level alterations, and diet.
The second function is called ADK shuttle. Effectively, adenylate kinase is a crucial figure in myofibrillar and mitochondrial areas of the cell. As such, it makes two high-energy phosphoryl of ATP, which can be moved between different adenine nucleotide molecules. Simply put; ADK acts as a shuttle that transfers ATP to the high energy consumption sites, then gets rid of AMP generated through these reactions. This process also leads to changes in the local intracellular metabolic flux - but it doesn’t create any alterations in global changes in metabolic concentrations. ADK shuttle is a function that’s imperative for general homeostasis of a cell.