|
Coagulation factor V, Activated protein C cofactor, FVL, PCCF, factor V, APC.
Activated Protein C exerts an antithrombotic effect by inhibiting Factors Va and VIIIa.
In vitro data indicate that Activated Protein C has indirect profibrinolytic activity through its ability to inhibit plasminogen activator inhibitor-1 (PAI-1) and limiting generation of activated thrombin-activatable-fibrinolysis-inhibitor. Additionally, in vitro data indicate that Activated Protein C may exert an anti-inflammatory effect by inhibiting human tumor necrosis factor production by monocytes, by blocking leukocyte adhesion to selectins, and by limiting the thrombin-induced inflammatory responses within the microvascular endothelium.
Activated Protein C Human Recombinant produced in CHO is a disulfide-linked homodimeric, glycosylated, polypeptide chain having a molecular mass of 55kDa.
Activated Protein C Human Recombinant is a serine protease with the same amino acid sequence and glycosilation sites as the human plasma-derived Activated Protein C.
The APC is purified by proprietary chromatographic techniques.
Chinese Hamster Ovary Cells (CHO).
Sterile Filtered White lyophilized (freeze-dried) powder.
Each mg protein contains 7. 6mg sodium chloride, 1.5 mg citrate and 6 mg sucrose.
It is recommended to reconstitute the lyophilized Coagulation factor V in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Factor V although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution APC should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw
Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE..
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
|
