Acrp30 Human | ProSpec

Description

Our Price

Quantity

Shipping

Adiponectin Human

	2µg($50)
	10µg($130)
	100µg($1000)

Catalogue Number

CYT-024

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (Adipokine). The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.

Patent Rights

The sale and/or commercial use of Recombinant Adiponectin is prohibited in the United States of America (U.S.A).

Description

The Acrp30 Human protein contains 244 amino acid having a molecular mass of 26.42kDa. Native adiponectin is isolated from human pooled sera and contains all HMW, MMW and LMW fractions.

Source

Human pooled serum

Formulation

Acrp30 Human was filtered (0.05µm) and lyophilized from 0.5 mg/ml supplied in 20mM TRIS, 50mM NaCl and 1mM CaCl2, pH 7.5

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.

Stability

Store lyophilized Acrp30 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Human can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino Acid Sequence

MLLLGAVLLL LALPGHDQET TTQGPGVLLP LPKGACTGWM AGIPGHPGHN GAPGRDGRDG TPGEKGEKGD PGLIGPKGDI GETGVPGAEG PRGFPGIQGR KGEPGEGAYV YRSAFSVGLE TYVTIPNMPI RFTKIFYNQQ NHYDGSTGKF HCNIPGLYYF AYHITVYMKD VKVSLFKKDK AMLFTYDQYQ ENNVDQASGS VLLHLEVGDQ VWLQVYGEGE RNGLYADNDN DSTFTGFLLY HDTN

Quality Control Test

BCA - to determine content of the protein.
SDS PAGE - to determine purity of the protein.

Applications

Cell culture and/or animal studies, ELISA, Immunological methods and Western blotting

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.