About ASF1A / ASF1 Anti-Silencing Function 1:
ASF1A also known as anti-silencing functioning 1 is a protein. Found in humans, this protein is encoded by the ASF1A gene.
ASF1A Mechanism
It has been proposed that ASF Could physically present the free histones directly to CBP for the process of acetylation. Despite this, the mechanism used for AS1 to present the free histones is not currently understood. However, once it is incorporated within the chromatin, the nucleosomes which are carrying K56ac do have a far looser intrinsic structure.
ASF1 Anti-Silencing Function 1 Interactions
During research, ASF1 has been shown to interact with a number of other proteins. This includes CHAF1A, CHAF1B, TLK2, and TLK1.
ASF1A Function
The protein is remarkably similar to the anti-silencing function-1 gene that is found in yeast. This protein is a key part of the histone donor complex. It is part of the function in the nucleosome assembly. It does interact with both H4 and H3 and functions with the chromatin assembly. This occurs during both replications as well as DNA repair.
ASF1 Anti-Silencing Function 1 Structure
ASF1 has a structure that includes an N-terminal of 155 residues that come together to form a globular core. This does consist largely of a fold that is like immunoglobulin. There are acidic patches that are highly conserved and believed to mediate any interactions that occur with H3. The structure does also suggest a strand capture mechanism that occurs between ASf1-H3/H4. Here, the H4 will act as a lever using the tail to trigger the chromatin assembly and disassembly. This may be used everywhere by histone chaperones.