ANGPTL3 Human | ProSpec

Description

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Angiopoietin Like Protein 3 Human Recombinant

	2µg($50)
	10µg($130)
	1mg($4800)

Catalogue Number

CYT-248

Synonyms

Angiopoietin 5, ANGPT5, ANGPTL3, Angiopoietin Like Protein 3.

Introduction

ANGPTL3 and ANGPTL4 are angiopoietin-like proteins secreted and expressed mainly by the liver, their role being the regulation of triglyceride metabolism by inhibiting the lipolysis of triglyceride-rich lipoproteins. During different nutritional states (feeding/fasting) the levels of the circulating triglycerides are regulated by Angptl3 and Angptl4 through differential inhibition of Lipoprotein lipase (LPL) as shown by the experimental data. The molecular structure of ANGPTL3 is similar to that of the angiopoietins (vascular endothelial growth factors). Deletion mutants of human Angiopoietin 5 were used in order to demonstrate that the N-terminal domain (fragment 17-207) and not the C-terminal fibrinogen-like domain (fragment 207-460) increased the plasma triglyceride levels in mice.

Description

The ANGPTL3 Human Recombinant is produced with N-terminal fusion of His-Tag.
The Angiopoietin-like protein 3 His Tagged Fusion Protein is 26kDa containing 207 amino acid residues of the ANGPTL3 Human and 16 additional amino acid residues – His-Tag (underlined).
MRGSHHHHHH GMASHMSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLQQKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN QQHSQIKEIE NQLRRTSIQE PTEISLSSKP RAP.

Source

Escherichia Coli.

Specificity

The amino acid sequence of the ANGPTL3 Human recombinant is 100% homologous to the 17-223 amino acid sequence of the Human Angiopoietin-like proteins-3 precursor without signal sequence.

Purification Method

One-step procedure using affinity Ni-NTA chromatography.

Formulation

ANGPTL3 Human Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Purity

Angiopoietin 5 purity is greater than 95% as determined by SDS-PAGE.

Solubility

Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet of ANGPTL3 Human dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of Angiopoietin 5 is limited.

Stability

Store lyophilized ANGPTL3 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Angiopoietin 5 can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Applications

Western blotting, ELISA.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.