ALAT Human
Alanine Aminotransferase Human Recombinant

Synonyms:
ALT1, Glutamic-pyruvic transaminase 1, GPT 1, Glutamic-alanine transaminase 1, AAT1, ALT, ALAT, SGPT.

Introduction:
Alanine transaminase or ALT is a transaminaseenzyme.
ALT is found in serumand in various bodily tissues, but is most commonly associated with the liver; It catalyzes the transfer of an aminogroup from alanineto a-ketoglutarate, the products of this reversible transaminationreaction being pyruvateand glutamate:
alanine+ α-ketoglutarate⇌ pyruvate+ glutamate
It is commonly measured clinically as a part of a diagnostic liver function test, to determine liver health. It is also called serum glutamate pyruvate transaminase (SGPT) or alanine aminotransferase (ALAT). Diagnostically, it is almost always measured in units/litre (U/L).

Description:
Alanine Aminotransferase Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 54,479 Dalton.
The ALT is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Formulation:
The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate.

Stability:
AAT1 although stable at 10°C for 5 days, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity:
The specific activity was found to be 928 IU/mg.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.