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N-acyl-L-amino-acid amidohydrolase, ACY-1, ACY1D, ACYLASE, ACY1.
Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.
Aminoacylase-1 Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-408 and having a total molecular mass of 46.9 kda. ACY-1 contains T7 tag at N-terminus. ACY1 is purified by proprietary chromatographic techniques.
Escherichia Coli.
Sterile Filtered clear solution.
Aminoacylase-1 at a concentration of 0.1mg/ml in 10mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.
ACYLASE although stable at 14°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
• ELISA
• MS
• Inhibition Assays
• Western Blotting
Greater than 95.0% as determined by SDS-PAGE.
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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