ACAD8 Human | ProSpec

Description

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Quantity

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Acyl-Coenzyme A Dehydrogenase 8 Human Recombinant

	2µg($50)
	10µg($130)
	1mg($4800)

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Catalogue Number

ENZ-294

Synonyms

Acyl-CoA dehydrogenase family member 8 mitochondrial, ACAD-8, Isobutyryl-CoA dehydrogenase, Activator-recruited cofactor 42 kDa component, ARC42, FLJ22590.

Introduction

Acyl CoA dehydrogenase is the enzymeused to catalyzethe first step of ?-oxidationin Fatty acid metabolism.
Acyl-coenzyme A (CoA) dehydrogenases (ACADs) are a family of mitochondrial enzymes that catalyze the first dehydrogenation step in the bets-oxidation of fatty acyl-CoA derivatives. Several human ACADs exist and all ACADs catalyze the same initial dehydrogenation of the substrate at the beta-carbon atom and require electron transfer flavoprotein as an alectron acceptor. The predicted 415-amino acid ACAD8 protein contains many of the residues conserved in most other ACADs, including an active site glutamic acid residue and residues important for tetramer formation.

Description

Acyl-Coenzyme A Dehydrogenase 8 Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-415 and having a total molecular mass of 47.7 kda. ACAD8 contains T7 tag at the N-terminus.
ACAD-8 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

Acyl-Coenzyme A Dehydrogenase 8 at a concentration of 0.1mg/ml in 10mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3, 10mM DTT.

Stability

ACAD8 although stable at 4°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Applications

• ELISA.
• MS.
• Inhibition Assays.
• Western Blotting.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.